Composite
Part:BBa_K3002124:Design
Designed by: Marlene Schlosser Group: iGEM19_TU_Kaiserslautern (2019-10-21)
Level 1 – Wildtype-PETASE + cCA + sp20-His
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1069
Illegal PstI site found at 2042 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 249
Illegal PstI site found at 1069
Illegal PstI site found at 2042 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1069
Illegal PstI site found at 2042 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1069
Illegal PstI site found at 2042
Illegal NgoMIV site found at 804
Illegal NgoMIV site found at 831
Illegal NgoMIV site found at 2533 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.
Source
This part is a combination of the parts BBa_K3002027 (PAR-promoter), BBa_K3002006 (RPL23-Terminator), BBa_K3002028 (CDS of Wildtype-PETase), BBa_K3002007 (cCA), BBa_K3002018 (spSP20-His-tag), BBa_K3002302 (connector B1-B2), BBa_K3002303 (connector B2-B3), BBa_K3002304 (connector B4-B5) and BBa_K3002305 (connector B5-B6).